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By default, the Modern Authentication required for third-party SAML SSO to work with Microsoft 365 is enabled for Exchange Online, though this setting can be changed by administrators. Before you continue to set up SSO, you’ll want to verify that it is enabled in your organization. To read more on Modern Authentication and how it affects Office applications, see Microsoft's . 

Verify Modern Authentication is enabled

Using Exchange Online Powershell

1. On a Microsoft Windows X86-based computer, run PowerShell as an administrator.
2. and run the following:
   • Import-Module ExchangeOnlineManagement
   • Connect-ExchangeOnline -UserPrincipalName <UPN>
3. Verify Modern Authentication is turned on:

4. You should see results like this:

5. If Modern Authentication isn’t enabled, enable it:

Using Microsoft 365 admin center

1. Log in to the Microsoft 365 admin center.
2. Navigate to Settings > Org Settings > Modern Authentication. 
3. Verify Turn on modern authentication for Outlook 2013 for Windows and later (recommended) is checked. If not, select the checkbox.
4. Click Save.